A display substrate according to an embodiment of the present disclosure comprises a substrate and a plurality of pixel units on the substrate, at least one of the plurality of pixel units comprising a light-emitting unit, a photosensitive unit for detecting light emitted by the light-emitting unit, and an interlayer insulating layer between the photosensitive unit and the light-emitting unit, wherein the interlayer insulating layer comprises a groove, an orthogonal projection of which on the substrate does not coincide with an orthogonal projection of the photosensitive unit on the substrate, and the light-emitting unit covers the groove.