An automatic vehicle disinfection system for promoting biosecurity through application of a disinfectant to a vehicle transiting to and from an agricultural site or the like. The automatic vehicle disinfection system includes a pair of resilient drums, each drum including a riser tube having one or more disinfectant dispensing nozzles, and at least one of the drums further including a disinfectant reservoir, a resilient cross tube fluidly connecting the riser tubes and including a central spray unit having one or more disinfectant dispensing nozzles, a pressure pump configured to pump disinfectant from the disinfectant reservoir to the disinfectant dispensing nozzles, and a control assembly including a pressure pump activation trigger configured to activate the pressure pump.