Invention Grant
- Patent Title: Methods and compositions for modulating PrfA mediated virulence gene activation in Listeria spp
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Application No.: US15754288Application Date: 2016-09-01
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Publication No.: US10774390B2Publication Date: 2020-09-15
- Inventor: Daniel A. Portnoy , Jonathan L. Portman
- Applicant: The Regents of the University of California
- Applicant Address: US CA Oakland
- Assignee: The Regents of the University of California
- Current Assignee: The Regents of the University of California
- Current Assignee Address: US CA Oakland
- Agency: Bozicevic, Field & Francis LLP
- Agent Bret E. Field
- International Application: PCT/US2016/050004 WO 20160901
- International Announcement: WO2017/040859 WO 20170309
- Main IPC: A61K39/02
- IPC: A61K39/02 ; C12Q1/689 ; C07K14/195 ; A61K35/74 ; C12N15/74 ; C12N1/20 ; A61P31/04 ; A61K39/00
Abstract:
Methods of assessing expression of a PrfA mediated expression construct in a Listerial host cell are provided. Aspects of the methods include maintaining a Listerial host cell that includes a PrfA mediated expression construct in a defined medium containing a reducing agent and evaluating expression of a product from the expression construct to assess expression of the PrfA mediated expression construct. Also provided are methods of producing and using a Listerial vaccine, as well as defined media for use in such methods.
Public/Granted literature
- US20180237828A1 METHODS AND COMPOSITIONS FOR MODULATING PRFA MEDIATED VIRULENCE GENE ACTIVATION IN LISTERIA SPP. Public/Granted day:2018-08-23
Information query
