A test cable and a test method using the same are provided. The test cable includes a universal serial bus (USB) connector, a test terminal connector and a switch circuit. The USB connector is coupled to an electronic device and has a first pair of data pins and a second pair of data pins. The test terminal connector is coupled to a test fixture and has a pair of test data pins and a first control pin, and the test fixture is used for outputting a first control signal to the first control pin. The switch circuit is coupled to the first pair and second pair of data pins, the pair of test data pins and the first control pin, and couples the pair of test data pins to the first pair or second pair of data pins according to the first control signal.