A compensation method for pixel aging applicable to controlling circuit of a display device having a display panel is provided. The method includes: receiving a display content; predicting an aging of each of a plurality of pixels of the display panel resulting from the display content in order to obtain an aging prediction; generating a display data to compensate the display panel based on the aging prediction; and outputting the display content. Besides, a controlling circuit using the method for compensating a display device is also provided.