In the method and device of driving display panel, and display device provided in the embodiment of the present disclosure, the display panel is divided into n sub-regions, and an average gray scale value of original pixel cells in each sub-region is determined, a gray scale threshold value range corresponding to the sub-region is determined according to the average gray scale value of the sub-region when the average gray scale value is greater than a preset gray scale threshold value, a ratio of the total number of original pixel cells in the gray scale threshold value range to the total number of original pixel cells contained in the corresponding sub-region is determined, and gamma value of original pixel cell in the corresponding sub-region according to the ratio and a preset gamma value conversion relationship is reset.