The invention relates to an assembly for ultrasound-assisted manipulation at the female reproductive organs of large mammals, such as rhinoceros, elephant and hippopotamus, and to the use of the assembly. The assembly comprises a support pipe with an end element and a handle piece, a guide pipe provided in the support pipe, a puncture needle, an ultrasound head support provided at the distal end of the support pipe and having accomodators for an ultrasound head and the distal end of the guide pipe, wherein the axis of the ultrasound head and of the distal end of the guide pipe is in each case angled with respect to the horizontal axis of the support pipe such that the position of the tip of the puncture needle is in the ultrasound scan range of the ultrasound head.