The present disclosure has disclosed a light guide device, comprising an optical waveguide layer (100), and a reflection layer (110) one surface of which is connected with the optical waveguide layer, said one surface including a plurality of protrusions (113), each protrusion including a first surface (114) forming a first angle (θ1) with respect to a surface of the optical waveguide layer, and a first intersection line (116) between a plane in which the surface of the optical waveguide layer is located and a plane in which the first surface is located being perpendicular to a light guiding direction of the optical waveguide layer. The present disclosure has disclosed a manufacturing method of a light guide device as well as a backlight module and display device including the light guide device at the same time.