The methods and system allow for the generation of a biometric electronic signature token using a subsequent biometric sample after an enrollment of a biometric reference value in a biometric system. The biometric electronic signature token is such that the data would be signed data, cryptographic message type, to package this signature, providing two authentication factors: the possession of the private key associated with the public/private key pair used to sign the signed data and the biometric sample of the user that could be matched against the template. The process allows a signing party to enroll in a biometric service, sign a piece of data or content using a public key, that may be tied to a trusted anchor certificate authority, and submit a biometric sample. Subsequently, the relying party may validate the information on that piece of data or content to confirm the identity of the signing party.