A tool holding frame has a track base, a positioning board, and at least one positioning mount. The positioning board is disposed on the track base and has a positioning segment. The first positioning segment has multiple positioning blocks having curved side edges and multiple positioning portions. The at least one positioning mount is slidably and rotatably mounted on the track base. Each positioning mount has an annular positioning flange and a second positioning segment. The second positioning segment is selectively engaged with two of the positioning portions.