An ultra-deep reservoir stratified water sample and sediment core integrated artificial intelligence sampling device, comprising a sampling device main chamber, an attitude balance sensor, a propeller, a balance base, and a sampler body, wherein the attitude balance sensor and the sampler body are disposed inside the sampling device main chamber; the propeller is disposed outside the sampling device main chamber; and the balance base is located at a bottom end of the sampling device main chamber. The sampling device of the present invention is an intelligent sampling device integrating high-definition underwater topography observation, undisturbed sediment core collection, vertical stratified accurate sampling of water bodies, and real-time in-situ monitoring of key physical and chemical parameters, and can be flexibly applied to deep and shallow water environments under complicated conditions.