A display device includes a base substrate, a pixel defining layer, a spacer layer and a photosensitive circuit. The pixel defining layer is on the substrate and includes a pixel region and a pixel gap region; the spacer layer is on the pixel gap region of the pixel defining layer and at a side of the pixel defining layer away from the base substrate; and the photosensitive circuit is at a side of the pixel defining layer away from the spacer layer. The spacer layer is lightproof, a first opening is in the spacer layer, the first opening and the photosensitive circuit are overlapped with each other in a direction perpendicular to the base substrate, and the photosensitive circuit is configured to detect light passing through the first opening.