A dynamic Raman signal acquisition apparatus, system, and method involving: an excitation light source operable at a designated irradiation power and for a designated acquisition time for each Raman data acquisition; a Raman probe operatively associated with said excitation light source to irradiate the biological tissue at said designated irradiation power and for said designated acquisition time, and capture an optical Raman response therefrom; a spectrometer operable to spectrally analyze said optical Raman response; and a controller in operative communication with said excitation light source and said spectrometer to automatically adjust at least one signal acquisition parameter.