A display apparatus and a brightness uniformity compensation method are introduced. The display apparatus includes a display panel, a content analysis circuit, a compensation table generator and a pixel compensation circuit. The content analysis circuit receives display data for a pixel of the display panel and analyzes a display load of the display data to generate a data compensation value. The compensation table generator generates a compensation table that includes the data compensation value corresponding to the display data of each pixel of the display panel. The pixel compensation circuit compensates the display data with the corresponding data compensation value included in the compensation table to generate compensated display data, wherein the compensated display data are displayed on the display panel of the display apparatus.