Invention Grant
- Patent Title: Chimeric proteins which enhance the activity of DNA binding domains (DBD) and transcription factors in plants
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Application No.: US15537047Application Date: 2015-12-16
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Publication No.: US10822612B2Publication Date: 2020-11-03
- Inventor: Carla Schommer , Javier Palatnik , Juan Manuel Debernardi , Ramiro Esteban Rodriguez Virasoro
- Applicant: CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (CONICET) , UNIVERSIDAD NACIONAL DE ROSARIO
- Applicant Address: AR Buenos Aires AR Santa Fe
- Assignee: CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (CONICET),UNIVERSIDAD NACIONAL DE ROSARIO
- Current Assignee: CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (CONICET),UNIVERSIDAD NACIONAL DE ROSARIO
- Current Assignee Address: AR Buenos Aires AR Santa Fe
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- International Application: PCT/IB2015/059696 WO 20151216
- International Announcement: WO2016/098027 WO 20160623
- Main IPC: C12N15/82
- IPC: C12N15/82 ; C07K14/415

Abstract:
The present invention relates to a method of enhancing the activity of a transcription factor in a plant comprising expressing in said plant a chimeric protein comprising a GIF moiety comprising a GIF domain or fragment thereof, and a DNA binding domain (DBD) moiety. The chimeric proteins comprise a GIF moiety comprising a GIF domain or fragment thereof, or a polypeptide with an amino acid sequence having at least 85%, preferably at least 90%, even more preferably at least 95% sequence identity to the amino acid sequence of a GIF domain or fragment thereof; and a DBD moiety comprising the DBD or plant transcription factor whose activity enhancement is intended, or a binding protein which is capable of forming a tertiary complex with the DBD or transcription factor whose activity modification and/or enhancement is intended. The invention also relates to polynucleotides and polypeptides useful in the method of the invention.
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