A method for calibrating an ANC-enabled portable audio device having microphones plays continuously a calibration sound by a calibrated speaker of a test station separate from the device. For each microphone of all the microphones, a microphone calibration value is computed using a comparison of a predetermined level and a measured level of an audio signal transduced by the microphone in response to the continuously-played calibration sound. The calibration is done without using a microphone of the test station. A processing element of the device may be programmed to make the comparison and computation. The processing element also causes a speaker of the device to generate a second calibration sound, measures a second level while the computed calibration value is applied to one of microphones (e.g., error microphone), and computes a calibration value for the device speaker using a comparison of a predetermined level and the second level.