Invention Grant
- Patent Title: Tissue selective transgene expression
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Application No.: US15775030Application Date: 2016-11-10
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Publication No.: US10851388B2Publication Date: 2020-12-01
- Inventor: Jaya Krishnan , Jonathan Ward
- Applicant: GENOME BIOLOGICS UG
- Applicant Address: DE Frankfurt am Main
- Assignee: GENOME BIOLOGICS UG
- Current Assignee: GENOME BIOLOGICS UG
- Current Assignee Address: DE Frankfurt am Main
- Agency: JMB Davis Ben-David
- Priority: EP15194155 20151111; EP16169261 20160511
- International Application: PCT/EP2016/077351 WO 20161110
- International Announcement: WO2017/081204 WO 20170518
- Main IPC: C12N15/86
- IPC: C12N15/86 ; C12N15/11 ; C12N15/85 ; A01K67/027 ; C12Q1/6888

Abstract:
A method for expression of transcribable unit(s) in a target cell is provided. The method comprises the steps of: a) providing a target cell expressing a site-specific recombinase, b) providing a DNA vector characterized by a 5′ to 3′ vector sequence orientation. The DNA vector comprises a plurality of recombination units, wherein a single recombination unit comprises at least one transcribable unit and a first type and a second type of target site that are recognizable by the site-specific recombinase. Recombination can only occur between two target sites of the same type and the first type of target site is located at the 5′ start of the recombination unit and the second type of target site is located at the 3′ end of the recombination unit. For all recombination units comprised within the DNA vector, the orientation of all of the first type of target sites are the same, and the orientation of all of the second type of target sites are the same. Step c) comprises introducing the DNA vector into the target cell.
Public/Granted literature
- US20180334686A1 TISSUE SELECTIVE TRANSGENE EXPRESSION Public/Granted day:2018-11-22
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