An example playback device is configured to receive a first stream of audio comprising source audio content to be played back by the playback device and record, via one or more microphones of the playback device, an audio signal output by the playback device based on the playback device playing the source audio content. The playback device is also configured to determine a transfer function between a frequency-domain representation of the first stream of audio and a frequency-domain representation of the recorded audio signal, and then determine an estimated frequency response of the playback device based on a difference between (i) the transfer function and (ii) a self-response of the playback device, where the self-response of the playback device is stored in a memory of the playback device. Based on the estimated frequency response, the playback device is configured to determine an acoustic calibration adjustment and implement the acoustic calibration adjustment.