Systems, methods, and apparatus for conditioning bamboo or other types of vegetable cane are provided. In one aspect, the present technology provides a cane processing assembly for use with a substantially flattened sheet of bamboo culm or vegetable cane having a plurality of longitudinally oriented fiber bundles. The assembly comprises first and second conditioning rollers configured to apply pressure to the sheet that breaks at least some natural bonds connecting laterally adjacent fiber bundles in the sheet. The first and second conditioning rollers are adjustable relative to each other so as to vary the pressure applied to the sheet by the first and second conditioning rollers.