Invention Grant
- Patent Title: DNA plasmids for the fast generation of homologous recombination vectors for cell line development
-
Application No.: US16568771Application Date: 2019-09-12
-
Publication No.: US10883120B2Publication Date: 2021-01-05
- Inventor: Oscar M. Perez-Leal
- Applicant: Temple University-Of The Commonwealth System of Higher Education
- Applicant Address: US PA Philadelphia
- Assignee: Temple University-Of The Commonwealth System of Higher Education
- Current Assignee: Temple University-Of The Commonwealth System of Higher Education
- Current Assignee Address: US PA Philadelphia
- Agency: Riverside Law LLP
- Main IPC: C12N15/90
- IPC: C12N15/90 ; C12N5/07 ; C12N5/16 ; C12N15/85
Abstract:
The present invention provides homologous recombination vectors to insert transgenic DNA in cells. These vectors shorten the production time and allow for easy generation of genetically modified cells. The invention allows the user to test multiple tags and to generate homozygous modified cell line using the homologous recombination vector. The invention can be used to generate knockout cells, to generate cell lines with knockin genes, to generate cell lines for drug screening against any target, to create transgenic animals, or in gene therapy.
Public/Granted literature
- US20200002729A1 DNA plasmids for the Fast Generation of Homologous Recombination Vectors For Cell Line Development Public/Granted day:2020-01-02
Information query
IPC分类:
