Invention Grant
- Patent Title: Use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants
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Application No.: US13132006Application Date: 2008-11-28
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Publication No.: US10894964B2Publication Date: 2021-01-19
- Inventor: Renata Fuganti , Alexandre Lima Nepomuceno , Francismar Correa Marcelino , Joao Flavio Veloso Silva , Silvana Regina Rochenbach Marin , Cesar Augusto Silveira , Eliseu Binneck
- Applicant: Renata Fuganti , Alexandre Lima Nepomuceno , Francismar Correa Marcelino , Joao Flavio Veloso Silva , Silvana Regina Rochenbach Marin , Cesar Augusto Silveira , Eliseu Binneck
- Applicant Address: BR Londrina; BR Londrina; BR Londrina; BR Londrina; BR Londrina; BR Londrina; BR Londrina
- Assignee: Renata Fuganti,Alexandre Lima Nepomuceno,Francismar Correa Marcelino,Joao Flavio Veloso Silva,Silvana Regina Rochenbach Marin,Cesar Augusto Silveira,Eliseu Binneck
- Current Assignee: Renata Fuganti,Alexandre Lima Nepomuceno,Francismar Correa Marcelino,Joao Flavio Veloso Silva,Silvana Regina Rochenbach Marin,Cesar Augusto Silveira,Eliseu Binneck
- Current Assignee Address: BR Londrina; BR Londrina; BR Londrina; BR Londrina; BR Londrina; BR Londrina; BR Londrina
- Agency: Sughrue Mion, PLLC
- International Application: PCT/BR2008/000361 WO 20081128
- International Announcement: WO2010/060162 WO 20100603
- Main IPC: C12N15/82
- IPC: C12N15/82 ; C07K14/415

Abstract:
The present invention refers to the use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants. The expression levels of the heat shock protein (Gmhsp17.6-L), when compared in resistant and susceptible individuals in the population, demonstrated that the largest expression levels per quantitative PCR were present in the individuals that contained the largest AT insertions in the promoter region. The invention also refers to gene expression cassettes containing promoter regions of the gene with different numbers of AT insertions fused to the GUS protein, for transforming soybean embryos.
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