Systems, devices, and methods are described herein for performing digital holography to analyze dynamics of fluid flow. According to some aspects of this disclosure, a Digital Fresnel Reflection Holography (DFRH) system, which is arranged to utilize light backscattered from particles in a fluid chamber to create a hologram that may be processed to analyze characteristics of fluid flow. The DFRH system may utilize light reflected from an imaging window disposed between a light source and a sampling volume, to be analyzed as a reference wave, to form an interference pattern and resultant hologram. According to some aspects of this disclosure, the DFRH techniques may provide simple, cost-effective mechanisms with improved performance over other techniques for analyzing fluid flow using holography.