Invention Grant
- Patent Title: High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
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Application No.: US15135377Application Date: 2016-04-21
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Publication No.: US10900073B2Publication Date: 2021-01-26
- Inventor: Paul C. Blainey , Michael W. Seely , Roman Stocker , Karsten Zengler , Scott Conradson , Peter Christey , Alexander Hallock
- Applicant: General Automation Lab Technologies, Inc.
- Applicant Address: US CA San Francisco
- Assignee: General Automation Lab Technologies, Inc.
- Current Assignee: General Automation Lab Technologies, Inc.
- Current Assignee Address: US CA San Francisco
- Agency: Liu Chen & Hoffman LLP
- Agent Lin Sun-Hoffman; Yong Chen
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H1/00 ; C12Q1/6888 ; C12Q1/6869 ; B01L3/00 ; C12Q1/6853 ; C12Q1/6816 ; C12Q1/689 ; C12M1/32 ; C12M1/04 ; B01L3/02

Abstract:
A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.
Public/Granted literature
- US20160312275A1 HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS FOR HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS Public/Granted day:2016-10-27
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