Invention Grant
- Patent Title: Double-strand DNA break quantification method
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Application No.: US16167781Application Date: 2018-10-23
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Publication No.: US10907199B2Publication Date: 2021-02-02
- Inventor: Marie-Chantal Gregoire , Guylain Boissonneault , Frédéric Leduc , Julien Massonneau , Olivier Simard , Chloé Lacombe-Burgoyne
- Applicant: SOCIETE DE COMMERCIALISATION DES PRODUITS DE LA RECHERCHE APPLIQUÉE SOCPRA
- Applicant Address: CA Sherbrooke
- Assignee: SOCIETE DE COMMERCIALISATION DES PRODUITS DE LA RECHERCHE APPLIQUÉE SOCPRA
- Current Assignee: SOCIETE DE COMMERCIALISATION DES PRODUITS DE LA RECHERCHE APPLIQUÉE SOCPRA
- Current Assignee Address: CA Sherbrooke
- Agent Marie-Helene Rochon
- Priority: CA2984019 20171027
- Main IPC: C12Q1/6851
- IPC: C12Q1/6851 ; C12Q1/686 ; C12Q1/6827
Abstract:
The present disclosure provides the quantification of double-strand breaks in DNA molecules using terminal deoxynucleotidyl transferase using a preliminary step of nick gap and repair. This preliminary step comprising contacting the DNA molecules with both a DNA ligase and a DNA polymerase to repair DNA nicks and remove DNA gaps prior to using the terminal deoxynucleotidyl transferase.
Public/Granted literature
- US20190161792A1 DOUBLE-STRAND DNA BREAK QUANTIFICATION METHOD Public/Granted day:2019-05-30
Information query
