Systems and methods for enhancing quality of a flow image of a sample are provided. An image is obtained from a plurality of optical image scans of a sample comprising blood perfused tissue. A vector comprising tissue components, flow components, and noise is then generated, and eigenvectors and eigenvalues are estimated from the vector. From the eigenvectors and eigenvalues, an eigen regression filter is applied to isolate flow components from the tissue components in the sample. The isolated tissue components may then be removed from the image to enhance visualization and quantification of a flow of dynamic moving particles within the sample.