A display substrate, a display device and a display control method are provided. The display substrate comprises a plurality of pixel units. Each of the plurality of pixel units includes a first region and a second region which is switchable between a non-opaque status and an opaque status. The first region comprises a first light-emitting unit which is a single-sided light-emitting unit. The second region includes at least one color-variable component covering the second region. Each of the at least one color-variable component is switchable between a transparent status and a non-transparent status. The second region is in the non-opaque status in a condition that the at least one color-variable component is in a transparent status. The second region is in the opaque status in a condition that the at least one color-variable component is in a non-transparent status.