It is aimed to prevent excitation light from leaking and being detected by the detector in a fluorescence microscope. A microscope includes an illumination optical system configured to illuminate a specimen with excitation light, a detector configured to detect fluorescence emitted from the specimen, and an observation optical system configured to guide the fluorescence to the detector. The observation optical system includes an optical filter having a variable wavelength characteristic, a light dispersing element configured to disperse, into light beams, light that has passed through the optical filter, and a light block plate configured to block at least some of the light beams resulting from the dispersion by the light dispersing element.