There is provided a method for image segmentation and annotation. The method comprises: providing an image; reducing the image size and smoothing the image to perform low-pass filtering on the image; restoring the image size; performing pixel level clustering of the image; performing spatial clustering of the image; selecting one or more clusters corresponding to a region of interest, ROI, based on a predetermined image mask, wherein the predetermined image mask has a known location in relation to the ROI; defining the selected one or more clusters as a proposed ROI; and determining if the proposed ROI sufficiently corresponds to an actual ROI.