The present disclosure relates to a mobile advertisement system and a method of advertisement using the mobile advertisement system. In certain embodiments, mobile advertisement system includes: a mobile advertisement server and one or more mobile advertisement delivery systems. The mobile advertisement server registers many advertisers and subscribers, receives various advertisements and their corresponding delivery instructions from advertisers, and dispatch advertisements and delivery instructions to the subscribers. Each of mobile advertisement delivery systems corresponds to one of registered subscribers, and is connected to mobile advertisement server. Each mobile advertisement delivery systems receive advertisements and delivery instructions from mobile advertisement server, and displays advertisements on respective mobile advertisement display device according to corresponding delivery instructions. Each delivery instruction corresponds to an advertisement, and includes: delivery time and delivery location. Each mobile advertisement delivery systems displays advertisements when it is positioned in a region specified by delivery location and during delivery time.