Systems, methods, and computer-readable media are disclosed for automated gates to secure mobile carrier unit payloads. In one embodiment, an example system for a mobile carrier unit of a sortation system may include an automated gate configured to block an open end of the mobile carrier unit in a first position, and to unblock the open end of the mobile carrier unit in a second position, and a controller configured to control operation of the gate. The controller may be configured to determine that the conveyor belt is activated, cause the automated gate to move to the second position, determine that the item is off the conveyor belt, and cause the automated gate to move to the first position.