Invention Grant
- Patent Title: Recombinant vector carrying cellulose binding domain and method for isolating and purifying protein, using same vector
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Application No.: US16611966Application Date: 2018-05-10
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Publication No.: US10934558B2Publication Date: 2021-03-02
- Inventor: Inhwan Hwang , Eun Ju Sohn , Yongjik Lee
- Applicant: BIOAPPLICATIONS INC.
- Applicant Address: KR Gyeongsangbuk-do
- Assignee: BIOAPPLICATIONS INC.
- Current Assignee: BIOAPPLICATIONS INC.
- Current Assignee Address: KR Gyeongsangbuk-do
- Agency: Riverside Law LLP
- Priority: KR10-2017-0058882 20170511
- International Application: PCT/KR2018/005371 WO 20180510
- International Announcement: WO2018/208099 WO 20181115
- Main IPC: C12N15/82
- IPC: C12N15/82 ; C07K1/22 ; C07K1/30 ; C07K5/11 ; C07K14/195 ; C07K16/00 ; C07K14/35
Abstract:
The present invention relates to a recombinant vector carrying cellulose-binding domain 3 and a method for separating a target protein using the recombinant vector. The protein isolating method of the present invention can easily separate a fusion protein containing a target protein by using the recombinant vector to bind a transformed plant body to cellulose and can effectively isolate the target protein from a cellulose-binding domain by treating the fusion protein with enterokinase, thus being expected to find industrial applications in various fields.
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