The present disclosure provides devices, systems and methods for automated processing of biological samples. This disclosure provides a gel-frame cassette for an automated bioprocessing device, comprising: a gel-frame comprising: a front face, a back face, a frame comprising: two vertical side bars, each comprising a gel-frame holding hole, a top bar connecting the two vertical side bars, a bottom bar connecting the two vertical side bars and a hollow chamber enclosed by the two vertical side bars, the top bar, and the bottom bar; a front panel in contact with the front face of the gel-frame, the front panel comprising an expanded upper portion, and a back panel in contact with the back face of the gel-frame, the back panel comprising a horizontal opening at the bottom of the back panel. This disclosure also provides an automated bioprocessing machine that processes the gel-frame cassette or the gel-frame.