A pre-charge circuit of a static random access memory (SRAM) controller and a pre-charging method thereof are provided. The pre-charge circuit of the SRAM controller includes a first switch, a second switch and a third switch. A first terminal of the first switch is coupled to a working voltage, a second terminal of the first switch is coupled to a first bit line of the SRAM controller, and the first switch is controlled by a first turn-on signal. A first terminal of the second switch is coupled to the working voltage, a second terminal of the second switch is coupled to a second bit line of the SRAM controller, and the second switch is controlled by a second turn-on signal. The third switch is coupled between the first bit line and the second bit line, and the third switch is controlled by a third turn-on signal. In a pre-charge mode, a time point at which the third switch is turned on is earlier than a time point at which the first switch is turned on and earlier than a time point at which the second switch is turned on.