In accordance with some embodiments, a fluid sample collection and retrieval apparatus including a microfluidic chip, a retrieval tube, a first switch, a second switch and a processor is provided. The microfluidic chip includes a first sample channel, a first fluid directing channel assembly, a first confluence chamber, a first collection channel, a first waste channel, and a retrieval hole. The retrieval hole passes through an outer surface of the microfluidic chip. The retrieval tube is connected to the retrieval hole. The first switch is connected to the microfluidic chip. The second switch is attached to the retrieval tube. The processor is configured to activate the first switch to operate the flow adjustment of the first fluid directing channel assembly and activate the second switch to operate a sample collection in the first collection channel within duration of operating the flow adjustment of the first fluid directing channel assembly.