A collection bottle and a method for detecting a characteristic of a sample are provided. The collection bottle includes a body and a cover. The body of the collection bottle has an accommodating space. The cover of the collection bottle is removably assembled to the body of the collection bottle, and the cover includes a channel and a testing part. The channel of the collection bottle communicates the accommodating space and the outside environment. While the cover of the collection bottle is assembled to the body, the testing part extends toward the accommodating space.