Waste transfer system for a toilet of a public transport vehicle that discharges into an on-board waste aerated storage tank, comprising a waste intermediate tank (1), means for generating negative or positive pressure inside said waste intermediate tank (1), and a discharge valve means (3,5,6,7) to regulate the flow through a first waste passage (8) between a toilet bowl (2) and said waste intermediate tank (1) and through a second waste passage (11) between said waste intermediate tank (1) and said waste storage tank, characterized in that said discharge valve means comprise a multi-port discharge valve (3) connectable to an inlet/outlet port (4) of the waste intermediate tank (1), said multi-port discharge valve (3) being configured to provide, in a first working position, direct communication between said toilet bowl (2) and said waste intermediate tank (1) when negative pressure is supplied inside said waste intermediate tank (1) and to provide, in a second working position, direct communication between the waste intermediate tank (1) and the waste storage tank when positive pressure is supplied in the said waste intermediate tank (1).