The present disclosure describes systems and methods for iris liveness determination. A sensor can acquire a first image of an iris with pupillary constriction, and a second image of the iris without the pupillary constriction. A processor can extract an iris portion of the first image into a first iris image, and an iris portion of the second image into a second iris image. The processor can normalize the first iris image and the second iris image. The normalized first iris image can have same dimensions as that of the normalized second iris image, in pixels. The processor can compute a minimum average difference between values of specific pixels of the normalized first iris image and values of corresponding pixels of the normalized second iris image. The specific pixels can correspond to an innermost annular portion of the iris.