The present invention provides an evaluation method of evaluating a measurement condition of a position of a mark formed on a substrate, the method comprising: obtaining a mark signal representing an intensity distribution of reflected light by detecting the reflected light from the mark under the measurement condition; generating a plurality of signals from the mark signal by changing a first signal component of a first frequency included in the mark signal obtained in the obtaining; and estimating a position of the mark from each of the plurality of signals obtained in the generating, and obtaining a variation in estimated position of the mark as an evaluation index of the measurement condition.