The present disclosure is directed to a novel system for using unique device and user identifiers to perform authentication of a user, device, and/or transaction. In particular, the system may use device biometric profiles and/or user identifiers to generate a unique identifiable signature for each user and/or device. The unique signature may then be used to authenticate devices as well as transactions submitted by said devices. In this way, the system increases the security of device authentication by helping to prevent the use of device hijacking methods that circumvent conventional authentication practices.