Invention Grant
- Patent Title: Media and fermentation methods for producing polysaccharides in bacterial cell culture
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Application No.: US15775172Application Date: 2016-11-10
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Publication No.: US10947494B2Publication Date: 2021-03-16
- Inventor: Sunil Gururao Desai , Michael Allen Hanson , Jonathan Patrick Kinross , Daniel R. Lasko , Scott Ellis Lomberk , Jason Arnold Lotvin , Sujata Kaushikbhai Patel-Brown , Weiqiang Sun , Peter Anthony Tomasello
- Applicant: Pfizer Inc.
- Applicant Address: US NY New York
- Assignee: Pfizer Inc.
- Current Assignee: Pfizer Inc.
- Current Assignee Address: US NY New York
- Agent Carol A. McKeever
- International Application: PCT/IB2016/056780 WO 20161110
- International Announcement: WO2017/085602 WO 20170526
- Main IPC: C12N1/20
- IPC: C12N1/20 ; C12P19/04 ; C12R1/46
Abstract:
The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide-producing bacteria.
Public/Granted literature
- US20190292513A1 MEDIA AND FERMENTATION METHODS FOR PRODUCING POLYSACCHARIDES IN BACTERIAL CELL CULTURE Public/Granted day:2019-09-26
Information query
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