Invention Grant
- Patent Title: Yeast strains co-expressing exogenous glucoamylases, the method for obtaining said yeast strains and the use thereof to produce bioethanol
-
Application No.: US15755675Application Date: 2016-08-23
-
Publication No.: US10947519B2Publication Date: 2021-03-16
- Inventor: Maud Petit , Georges Pignede , Jean-Michel Bavouzet , Benoît Thomas
- Applicant: LESAFFRE ET COMPAGNIE
- Applicant Address: FR Paris
- Assignee: LESAFFRE ET COMPAGNIE
- Current Assignee: LESAFFRE ET COMPAGNIE
- Current Assignee Address: FR Paris
- Agency: Duane Morris LLP
- Agent Thomas J. Kowalski; Deborah L. Lu
- Priority: FR1558079 20150831
- International Application: PCT/FR2016/052107 WO 20160823
- International Announcement: WO2017/037362 WO 20170309
- Main IPC: C12N9/34
- IPC: C12N9/34 ; C12N15/81 ; C12P7/06

Abstract:
The present application relates to improved Saccharomyces cerevisiae yeast strains that co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus. The present invention also relates to a method for obtaining these yeast strains involving a) genetically modifying a Saccharomyces cerevisiae yeast to co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus; b) culturing and fermenting the strain obtained in step a) on a dextrin medium; c) selecting the strains having fermentation kinetics at least equal to or greater than those obtained with the strain deposited at the CNCM [French National Collection of Microorganism Cultures] under number I 4999 under the same conditions. The yeast strains according to the invention are of particular interest in producing bioethanol.
Public/Granted literature
Information query
IPC分类: