- Patent Title: Method for producing lactic acid in recombinant yeast cell culture
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Application No.: US16094186Application Date: 2017-04-14
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Publication No.: US10947571B2Publication Date: 2021-03-16
- Inventor: Magnus Ask , Rakesh Koppram , Diethard Mattanovich , Michael Sauer
- Applicant: Syconium Lactic Acid GmbH
- Applicant Address: AT Vienna
- Assignee: Syconium Lactic Acid GmbH
- Current Assignee: Syconium Lactic Acid GmbH
- Current Assignee Address: AT Vienna
- Agency: Loza & Loza, LLP
- Agent Michael F. Fedrick
- Priority: EP16165772 20160418
- International Application: PCT/EP2017/059046 WO 20170414
- International Announcement: WO2017/182403 WO 20171026
- Main IPC: C12P7/56
- IPC: C12P7/56 ; C12N1/18 ; C12N15/52 ; C07K14/395 ; C12N9/04 ; C12N9/88

Abstract:
The present invention provides a method for producing lactic acid in a recombinant yeast cell culture using glucose as carbon source comprising a first, seed fermentation stage to produce biomass wherein the yeast is cultivated in a culture medium at a pH of 5 to 7, followed by a second, a production fermentation stage with biomass from the seed fermentation to produce lactic acid, wherein the yeast is cultivated in a culture medium at low p H using a yeast strain that is engineered to have lactate dehydrogenase (LDH) activity and optionally has decreased or knocked-out pyruvate decarboxylase (PDC) activity.
Public/Granted literature
- US20190127766A1 METHOD FOR PRODUCING LACTIC ACID Public/Granted day:2019-05-02
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