A capacitive sensing and sampling circuit and method thereof are disclosed. The capacitive sensing and sampling circuit has a voltage source, a sensing unit and a detecting circuit. The detecting unit has a first sensing output unit, a second sensing output unit and a sampling unit. The first and second sensing output units are electrically connected to an output of the sensing unit and the sampling unit. Therefore, by increasing the number of alternatively sensing the capacitive sensing component to obtain more capacitive signals, the number of sampling the sensed capacitive signal is relatively increased. Therefore, the white noise interference for the capacitive sensing and sampling circuit is effectively suppressed, so the signal-to-noise ratio is increased and accuracy of detecting coordinates of the touch object is increased.