Provided herein is a method of manufacturing a semiconductor device. The method for manufacturing the semiconductor device includes: alternately stacked first material layers and second material layers on a lower structure; forming first holes passing through the first material layers and the second material layers, each of the first holes defining a channel region; removing the second material layers through the first holes such that interlayer spaces between the first material layers are formed; and forming, through the first holes, conductive patterns which fill respective interlayer spaces.