A hamstring exercise device is provided that allows a user to apply variable resistance to the hamstring muscles during exercise. The hamstring exercise device has a user sled, upon which a user may rest, slidably mounted on an angulation slide. The angulation slide is pivotably adjustable to varying degrees of inclination or declination relative to the horizontal. The user's body weight, resistance of the sled toward moving and an optional biasing means all contribute to an adjustable degree of exercise difficulty.