The present invention provides a line over driving (LOD) table adjustment method and a system thereof, which divides a display panel into M*N blocks. In an i-th row and a j-th column in the LOD table corresponding to a block, according to a brightness value of a grayscale value of a pixel of a previous line and a current line corresponding to an initial grayscale value of over driving pixels, calculate a target brightness value of an adjustment screen corresponding to a combination of the grayscale value of the pixel of the previous line and the current line corresponding to the initial grayscale value of over driving pixels, and obtain a measured brightness value of the adjustment screen to compare whether the difference between the target brightness value and the measured brightness value is within the default error range value to determine a final grayscale value of over driving pixel.