A bone-meat separation device for a bone-in limb meat according to an embodiment is a bone-meat separation device for a bone-in limb meat for separating a meat part adhering to a trunk-side end bone head of a bone-in limb meat, the device including a plurality of clamp portions for conveying the bone-in limb meat along a conveying path, a guide portion disposed on the conveying path and forming a V-shaped guide space with an open end thereof being directed upstream in a conveying direction of the bone-in limb meat, a cutter disposed at a downstream end of the guide space in the conveying direction, and a holding portion holding the meat part below the guide portion and the cutter. The holding portion is configured to hold the meat part of the bone-in limb meat when the meat part is cut from the trunk-side end bone head with the cutter.