Provided is a system and method for an anti-foaming needle assembly for avoiding foam generation when extracting a liquid from a vessel having a chamber with a bottom and opposite thereto an opening sealed with a penetrable and re-sealable stopper providing an inner surface above the chamber. More specifically, the anti-foaming needle assembly includes a needle base having: a first end and opposite thereto a second end and a sidewall there between, the second end structured and arranged for temporary connection to a syringe having a barrel and a plunger; a first needle having a hollow shaft and a first length extending from the first end to provide an open distal end above the first end, the first needle having a second end vented through the sidewall; and a second needle having a hollow shaft and a second length extending from the first end generally parallel to the first needle to provide, when inverted, a distal end above the first end and below the distal end of the first needle, the second needle having a second end in fluid communication with the second end of the needle base. An associated method of use is also provided.