A body frame of a two-wheeled motor vehicle includes a first frame extending rearward from a head pipe, a second frame disposed beneath the first frame with a gap there between, a third frame disposed beneath the second frame with a gap there between, a plate-shaped first reinforcing member provided between the first frame and the second frame, and a plate-shaped second reinforcing member provided between the second frame and the third frame, and the first reinforcing member and the second reinforcing member are formed so that external shapes thereof are connected. Thus, it is possible to provide a body frame for a two-wheeled motor vehicle that contributes to cutting the number of components and increasing the stiffness.