- Patent Title: Systems and methods for immobilizing extracellular matrix material on organ on chip, multilayer microfluidics microdevices, and three-dimensional cell culture systems
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Application No.: US15748066Application Date: 2016-07-27
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Publication No.: US11008546B2Publication Date: 2021-05-18
- Inventor: Dongeun Huh , Mark Mondrinos , Cassidy Blundell , Jeongyun Seo
- Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
- Applicant Address: US PA Philadelphia
- Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
- Current Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
- Current Assignee Address: US PA Philadelphia
- Agency: Baker Botts, L.L.P.
- International Application: PCT/US2016/044321 WO 20160727
- International Announcement: WO2017/019799 WO 20170202
- Main IPC: C12N5/00
- IPC: C12N5/00 ; A61L27/22 ; A61L27/18 ; A61L27/38 ; C12M3/00 ; C12M1/00 ; C12M1/12 ; A61L27/52 ; C08L83/04 ; A61L27/36
Abstract:
The presently disclosed subject matter provides an approach to address the needs for microscale control in shaping the spacial geometry and microarchitecture of 3D collagen hydrogels. For example, the disclosed subject matter provides for compositions, methods, and systems employing N-sulfosuccinimidyl-6-(4′-azido-2′-nitro-phenylamino)hexanoate (“sulfo-SANPAH”), to prevent detachment of the hydrogel from the anchoring substrate due to cell-mediated contraction.
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